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PAK4/PAK5/PAK6(Phospho Ser474/S560/S602) (AB4451) Rabbit mAb
PAK4/PAK5/PAK6(Phospho Ser474/S560/S602) (AB4451) Rabbit mAb

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Catalog No: M1246
Species Reactivity:Mouse,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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PAK4/PAK5/PAK6(Phospho Ser474/S560/S602) (AB4451) Rabbit mAb

Western blot analysis of various lysates using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (M1246) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

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Product Details
Product Name
PAK4/PAK5/PAK6(Phospho Ser474/S560/S602) (AB4451) Rabbit mAb
Description
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Catalog No
M1246
Species Reactivity
Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S474/S560/S602
Clone
AB4451
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S474 of human PAK4PAK4 (O96013).
Specificity
The antibody detects endogenous levels of PAK4/PAK5/PAK6 protein only when phosphorylated at Ser474/Ser560/Ser602. The antibody does not cross-react with PAK4/PAK5/PAK6 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
PAK4/PAK5/PAK6
Gene ID
10298; 57144; 56924
Human swiss
O96013(PAK4-Antigen); Q9P286(PAK5); Q9NQU5(PAK6)
Protein Name
PAK4/PAK5/PAK6(Phospho Ser474/S560/S602)
Synonyms
PAK4; KIAA1142; PAK5KIAA1264; PAK6; Phospho-PAK4/PAK5/PAK6-S474/S560/S602
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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