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B-Raf (Phospho Thr401) (AB2771) Rabbit mAb
B-Raf (Phospho Thr401) (AB2771) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1242
Species Reactivity:Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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B-Raf (Phospho Thr401) (AB2771) Rabbit mAb

Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (M1242) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

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Product Details
Product Name
B-Raf (Phospho Thr401) (AB2771) Rabbit mAb
Description
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
Catalog No
M1242
Species Reactivity
Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T401
Clone
AB2771
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:1000-1:2000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T401 of rat B-Raf protein.
Specificity
The antibody detects endogenous levels of B-Raf protein only when phosphorylated at Thr401. The antibody does not cross-react with B-Raf phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
BRAF
Gene ID
673
Human swiss
P15056
Protein Name
B-Raf (Phospho Thr401)
Synonyms
NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; Phospho-B-Raf-T401
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

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