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Src (Phospho Tyr419) (AB4944) Rabbit mAb
Src (Phospho Tyr419) (AB4944) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1235
Species Reactivity:Human,Mouse
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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Src (Phospho Tyr419) (AB4944) Rabbit mAb

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (M1235) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

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Product Details
Product Name
Src (Phospho Tyr419) (AB4944) Rabbit mAb
Description
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Catalog No
M1235
Species Reactivity
Human, Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho Y419
Clone
AB4944
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around Y419 of human SRC.
Specificity
The antibody detects endogenous levels of Src protein only when phosphorylated at Tyr419. The antibody does not cross-react with Src phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
SRC
Gene ID
6714
Human swiss
P12931
Protein Name
Src (Phospho Tyr419)
Synonyms
ASV; SRC1; THC6; c-SRC; p60-Src; Phospho-Src-Y419
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

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