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PLK1 (Phospho Thr210) (AB4587) Rabbit mAb
PLK1 (Phospho Thr210) (AB4587) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1233
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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PLK1 (Phospho Thr210) (AB4587) Rabbit mAb

Western blot analysis of lysates from HT-29 cells, using Phospho-PLK1-T210 (M1233) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

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Product Details
Product Name
PLK1 (Phospho Thr210) (AB4587) Rabbit mAb
Description
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.
Catalog No
M1233
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T210
Clone
AB4587
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T210 of human PLK1.
Specificity
The antibody detects endogenous levels of PLK1 protein only when phosphorylated at Thr210. The antibody does not cross-react with PLK1 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
PLK1
Gene ID
5347
Human swiss
P53350
Protein Name
PLK1 (Phospho Thr210)
Synonyms
PLK; STPK13; Phospho-PLK1-T210
Related Products

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HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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