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eIF4E(Phospho Ser209) (AB3381) Rabbit mAb
eIF4E(Phospho Ser209) (AB3381) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1232
Species Reactivity:Human,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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eIF4E(Phospho Ser209) (AB3381) Rabbit mAb

Western blot analysis of lysates from HeLa cells, using Phospho-eIF4E-S209 Rabbit mAb (M1232) at 1:1000 dilution.HeLa cells were treated by 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.

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Product Details
Product Name
eIF4E(Phospho Ser209) (AB3381) Rabbit mAb
Description
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Catalog No
M1232
Species Reactivity
Human, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB3381
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:1000-1:5000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A phospho specific peptide corresponding to residues surrounding S209 of human Phospho - eIF4E - S209.
Specificity
This antibody detects endogenous levels of eIF4E(Phospho Ser209) protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
EIF4E
Gene ID
1977
Human swiss
P06730
Protein Name
eIF4E(Phospho Ser209)
Synonyms
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1; Phospho-eIF4E-S209
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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