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Primary Antibodies
MEK1(Phospho Ser298) (AB4160) Rabbit mAb
MEK1(Phospho Ser298) (AB4160) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1229
Species Reactivity:Human,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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MEK1(Phospho Ser298) (AB4160) Rabbit mAb

Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (M1229) at 1:1000 dilution.Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37℃ for 1 hour or treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1min.

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Product Details
Product Name
MEK1(Phospho Ser298) (AB4160) Rabbit mAb
Description
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs) , act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra-and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Catalog No
M1229
Species Reactivity
Human, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S298
Clone
AB4160
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:1000-1:2000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S298 of human Phospho - MEK1 - S298 (Q02750).
Specificity
The antibody detects endogenous levels of MEK1 protein only when phosphorylated at Ser298. The antibody does not cross-react with MEK1 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
MAP2K1
Gene ID
5604
Human swiss
Q02750
Protein Name
MEK1(Phospho Ser298)
Synonyms
MEL; Phospho-MEK1-S298
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
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Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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