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CDK2 (Phospho Tyr15) (AB3041) Rabbit mAb
CDK2 (Phospho Tyr15) (AB3041) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1221
Species Reactivity:Mouse
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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CDK2 (Phospho Tyr15) (AB3041) Rabbit mAb

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK2-Y15 Rabbit mAb (M1221) at 1:1000 dilution.NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3s.

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Product Details
Product Name
CDK2 (Phospho Tyr15) (AB3041) Rabbit mAb
Description
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) , and p27Kip1 (CDKN1B) . Alternative splicing results in multiple transcript variants.
Catalog No
M1221
Species Reactivity
Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho Y15
Clone
AB3041
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around Y15 of mouse CDK2.
Specificity
The antibody detects endogenous levels of CDK2 protein only when phosphorylated at Tyr15. The antibody does not cross-react with CDK2 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
CDK2
Gene ID
1017
Human swiss
P24941
Protein Name
CDK2 (Phospho Tyr15)
Synonyms
CDKN2; p33(CDK2) ; Phospho-CDK2-Y15
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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