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Primary Antibodies
AMPKα1(Phospho Ser496) (AB2617) Rabbit mAb
AMPKα1(Phospho Ser496) (AB2617) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1218
Species Reactivity:Mouse
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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AMPKα1(Phospho Ser496) (AB2617) Rabbit mAb

Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb (M1218) at 1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 μM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

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Product Details
Product Name
AMPKα1(Phospho Ser496) (AB2617) Rabbit mAb
Description
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Catalog No
M1218
Species Reactivity
Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S496
Clone
AB2617
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:2000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S496 of mouse AMPKα1 (Q13131).
Specificity
The antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at Ser496. The antibody does not cross-react with AMPKα1 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
PRKAA1
Gene ID
5562
Human swiss
Q13131
Protein Name
AMPKα1(Phospho Ser496)
Synonyms
AMPK; AMPKa1; AMPK alpha 1; Phospho-AMPKα1-S496
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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