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Cdk1/2 (Phospho Thr14) (AB3038) Rabbit mAb
Cdk1/2 (Phospho Thr14) (AB3038) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1217
Species Reactivity:Human,Mouse,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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Cdk1/2 (Phospho Thr14) (AB3038) Rabbit mAb

Western blot analysis of various lysates using Phospho-Cdk1/2-T14 Rabbit mAb (M1217) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

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Product Details
Product Name
Cdk1/2 (Phospho Thr14) (AB3038) Rabbit mAb
Description
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF) , which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Catalog No
M1217
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T14
Clone
AB3038
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1.
Specificity
The antibody detects endogenous levels of Cdk1/2 protein only when phosphorylated at Thr14. The antibody does not cross-react with Cdk1/2 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
CDK1/CDK2
Gene ID
983; 1017
Human swiss
P06493; P24941
Protein Name
Cdk1/2 (Phospho Thr14)
Synonyms
CDC2; CDC28A; P34CDC2; Phospho-Cdk1/2-T14
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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