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IκBα(Phospho Ser36) (AB3983) Rabbit mAb
IκBα(Phospho Ser36) (AB3983) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1214
Species Reactivity:Human,Mouse,Rat
Application:WB,IHC-P,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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IκBα(Phospho Ser36) (AB3983) Rabbit mAb

Western blot analysis of various lysates using Phospho-IκBα-S36 Rabbit mAb (M1214) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight or treated by LPS (1 μg/mL) at 37℃ for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

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Product Details
Product Name
IκBα(Phospho Ser36) (AB3983) Rabbit mAb
Description
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Catalog No
M1214
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S36
Clone
AB3983
Isotype
IgG
Application
WB, IHC-P, ELISA
Application Dilutions
WB, 1:500-1:1000 | IHC-P, 1:50-1:200 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S36 of human IκBα.
Specificity
The antibody detects endogenous levels of IκBα protein only when phosphorylated at Ser36. The antibody does not cross-react with IκBα phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
NFKBIA
Gene ID
4792
Human swiss
P25963
Protein Name
IκBα(Phospho Ser36)
Synonyms
IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S36
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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