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Primary Antibodies
GCLC (AB3578) Rabbit mAb
GCLC (AB3578) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0921
Species Reactivity:Human,Rat
Application:WB,IHC-P,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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GCLC (AB3578) Rabbit mAb

Western blot analysis of lysates from Rat kidney, using GCLC Rabbit mAb (M0921) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.

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Product Details
Product Name
GCLC (AB3578) Rabbit mAb
Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Catalog No
M0921
Species Reactivity
Human, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB3578
Isotype
IgG
Application
WB, IHC-P, ELISA
Application Dilutions
WB, 1:500-1:1000 | IHC-P, 1:100-1:800 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 538 - 637 of human GCLC.
Specificity
This antibody detects endogenous levels of GCLC protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
GCLC
Gene ID
2729
Human swiss
P48506
Protein Name
GCLC
Synonyms
GCL; GCS; GLCL; GLCLC; GCLC
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

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