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Primary Antibodies
Smad3 (AB4899) Rabbit mAb
Smad3 (AB4899) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0571
Species Reactivity:Human,Mouse
Application:WB,IHC-P,IF/ICC,IP,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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Smad3 (AB4899) Rabbit mAb

Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using Smad3 Rabbit mAb (M0571) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

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Product Details
Product Name
Smad3 (AB4899) Rabbit mAb
Description
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Catalog No
M0571
Species Reactivity
Human, Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB4899
Isotype
IgG
Application
WB, IHC-P, IF/ICC, IP, ELISA
Application Dilutions
WB, 1:500-1:1000 | IHC-P, 1:50-1:200 | IF/ICC, 1:50-1:200 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 119 - 219 of human Smad3.
Specificity
This antibody detects endogenous levels of Smad3 protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.09% Sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
SMAD3
Gene ID
4088
Human swiss
P84022
Protein Name
Smad3
Synonyms
LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; d3
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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