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BTK (Phospho Tyr223) (AB2780) Rabbit mAb
BTK (Phospho Tyr223) (AB2780) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0470
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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BTK (Phospho Tyr223) (AB2780) Rabbit mAb

Western blot analysis of lysates from Raji cells, using Phospho-BTK-Y223 Rabbit mAb (M0470) at 1:500 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

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Product Details
Product Name
BTK (Phospho Tyr223) (AB2780) Rabbit mAb
Description
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.
Catalog No
M0470
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho Y223
Clone
AB2780
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around Y223 of human BTK (Q06187).
Specificity
The antibody detects endogenous levels of BTK protein only when phosphorylated at Tyr223. The antibody does not cross-react with BTK phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
BTK
Gene ID
695
Human swiss
Q06187
Protein Name
BTK (Phospho Tyr223)
Synonyms
AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1; Phospho-BTK-Y223
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
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Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
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