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Primary Antibodies
PKR/EIF2AK2 (Phospho Thr446) (AB4571) Rabbit mAb
PKR/EIF2AK2 (Phospho Thr446) (AB4571) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0462
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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PKR/EIF2AK2 (Phospho Thr446) (AB4571) Rabbit mAb

Western blot analysis of lysates from HeLa cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (M0462) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

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Product Details
Product Name
PKR/EIF2AK2 (Phospho Thr446) (AB4571) Rabbit mAb
Description
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
Catalog No
M0462
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T446
Clone
AB4571
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:2000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2E2AK2.
Specificity
The antibody detects endogenous levels of PKR/EIF2AK2 protein only when phosphorylated at Thr446. The antibody does not cross-react with PKR/EIF2AK2 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
EIF2AK2
Gene ID
5610
Human swiss
P19525
Protein Name
PKR/EIF2AK2 (Phospho Thr446)
Synonyms
PKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; Phospho-PKR/EIF2AK2-T446
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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