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ERK1 (Phospho Thr202) + ERK2 (Phospho Thr185) (AB3427) Rabbit mAb
ERK1 (Phospho Thr202) + ERK2 (Phospho Thr185) (AB3427) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0374
Species Reactivity:Human,Mouse,Rat
Application:WB,IHC-P,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
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ERK1 (Phospho Thr202) + ERK2 (Phospho Thr185) (AB3427) Rabbit mAb

Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (M0374) at 1:1000 dilution.Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

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Product Details
Product Name
ERK1 (Phospho Thr202) + ERK2 (Phospho Thr185) (AB3427) Rabbit mAb
Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21. 3.
Catalog No
M0374
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T202, Phospho T185
Clone
AB3427
Isotype
IgG
Application
WB, IHC-P, ELISA
Application Dilutions
WB, 1:500-1:2000 | IHC-P, 1:50-1:200 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T202 of human ERK1.
Specificity
The antibody detects endogenous levels of ERK1 protein only when phosphorylated at Thr202 and ERK2 protein when phosphorylated Thr185. The antibody does not cross-react with ERK1 and ERK2 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
ERK1/ERK2
Gene ID
5594; 5595
Human swiss
P28482; P27361
Protein Name
ERK1 (Phospho Thr202) + ERK2 (Phospho Thr185)
Synonyms
H3. 4; H3/g; H3FT; H3t; H3/A; H3FA; ERK1 / ERK2; Phospho-ERK1-T202 + ERK2-T185
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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