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Primary Antibodies
T-bet / Tbx21 (AB5023) Rabbit mAb
T-bet / Tbx21 (AB5023) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0270
Species Reactivity:Human,Mouse,Rat
Application:WB,IP,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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T-bet / Tbx21 (AB5023) Rabbit mAb

Western blot analysis of various lysates using T-bet / Tbx21 Rabbit mAb (M0270) at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC) : U-937|Exposure time: 180s.

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Product Details
Product Name
T-bet / Tbx21 (AB5023) Rabbit mAb
Description
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG) . Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Catalog No
M0270
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB5023
Isotype
IgG
Application
WB, IP, ELISA
Application Dilutions
WB, 1:500-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells | ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 322 - 535 of human T - bet/Tbx21.
Specificity
This antibody detects endogenous levels of T-bet / Tbx21 protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.09% Sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
TBX21
Gene ID
30009
Human swiss
Q9UL17
Protein Name
T-bet / Tbx21
Synonyms
TBET; IMD88; T-PET; T-bet; TBLYM
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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    • Research Areas

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