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Primary Antibodies
AKT2 (Phospho Ser474) (AB2580) Rabbit mAb
AKT2 (Phospho Ser474) (AB2580) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0188
Species Reactivity:Human
Application:WB,IF/ICC,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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AKT2 (Phospho Ser474) (AB2580) Rabbit mAb

Confocal imaging of 293F cells transfected with AKT2 using Phospho-AKT2-S474 (Akt2 Specific) Rabbit mAb (M0188, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (SA1138, dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.

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Product Details
Product Name
AKT2 (Phospho Ser474) (AB2580) Rabbit mAb
Description
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
Catalog No
M0188
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S474
Clone
AB2580
Isotype
IgG
Application
WB, IF/ICC, ELISA
Application Dilutions
WB, 1:1000-1:2000 | IF/ICC, 1:50-1:200 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S474 of human AKT2.
Specificity
The antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474. The antibody does not cross-react with AKT1 /AKT2/AKT3 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
AKT2
Gene ID
208
Human swiss
P31751
Protein Name
AKT2(Phospho Ser474(Akt2 Specific)
Synonyms
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; Phospho-AKT2-S474(Akt2 Specific)
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Primary Antibodies

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