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BRD4(Phospho Ser1083) (AB2774) Rabbit mAb
BRD4(Phospho Ser1083) (AB2774) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0114
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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BRD4(Phospho Ser1083) (AB2774) Rabbit mAb

Western blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb (M0114) at1:500 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 300s.

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Product Details
Product Name
BRD4(Phospho Ser1083) (AB2774) Rabbit mAb
Description
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19) (q13;p13. 1) , which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Catalog No
M0114
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S1083
Clone
AB2774
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S1083 of human BRD4.
Specificity
The antibody detects endogenous levels of BRD4 protein only when phosphorylated at Ser1083. The antibody does not cross-react with BRD4 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
BRD4
Gene ID
23476
Human swiss
O60885
Protein Name
BRD4(Phospho Ser1083)
Synonyms
CAP; MCAP; HUNK1; HUNKI; FSHRG4; Phospho-BRD4-S1083
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

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