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Primary Antibodies
Beclin 1 (AB2745) Rabbit mAb
Beclin 1 (AB2745) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0093
Species Reactivity:Human,Mouse,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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Beclin 1 (AB2745) Rabbit mAb

Western blot analysis of lysates from wild type (WT) and Beclin1 knockout (KO) 293T (KO) cells, using Beclin 1 Rabbit mAb (M0093) at 1:30000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

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Product Details
Product Name
Beclin 1 (AB2745) Rabbit mAb
Description
Beclin 1 plays a central role in autophagy. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. There two casp cleavage sites in Beclin 1, TDVD(133) and DQLD(149) , cleavage at which yields fragments lacking the autophagy-inducing capacity. Noteworthy, the C-terminal fragment, Beclin 1-C, localized predominantly at the mitochondria and sensitized the cells to apoptosis. It induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Catalog No
M0093
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB2745
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:10000-1:30000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1 - 280 of human Beclin 1.
Specificity
This antibody detects endogenous levels of Beclin 1 protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
Beclin 1
Gene ID
8678
Human swiss
Q14457
Protein Name
Beclin 1
Synonyms
ATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1;
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HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
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Size:
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Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
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Publications (10)
TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways. Cell Death & Disease Cell Death Dis. 2020 Jan;11(1):1-15 WB Rat heart

Author: Available in link provided

Date of Publication : Available in link provided

Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα and cell-cycle-driving proteins. Scientific Reports Sci Rep-Uk. 2016 Jan;6(1):1-10 IHC Mouse 1:100 mammary gland

Author: Available in link provided

Date of Publication : Available in link provided

Targeting FoxM1 by thiostrepton inhibits growth and induces apoptosis of laryngeal squamous cell carcinoma. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 2014 Nov 13 WB Human Hep-2 cell

Author: Available in link provided

Date of Publication : Available in link provided

TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-β/Smads and ERK1/2 signaling pathways. EBioMedicine Ebiomedicine. 2021 May;67:103370 WB Mouse 1 : 1000 Heart tissues CFs

Author: Available in link provided

Date of Publication : Available in link provided

Gene silencing of USP1 by lentivirus effectively inhibits proliferation and invasion of human osteosarcoma cells. INTERNATIONAL JOURNAL OF ONCOLOGY 2016 Dec 01 WB Human U2OS cell

Author: Available in link provided

Date of Publication : Available in link provided

The Catalytically Inactive Mutation of the Ubiquitin-Conjugating Enzyme CDC34 Affects its Stability and Cell Proliferation. PROTEIN JOURNAL Protein J. 2018 Apr;37(2):132-143 WB Human HEK293T cell, HeLa cell

Author: Available in link provided

Date of Publication : Available in link provided

Zhang, Peng, et al. "Tubulin cofactor A functions as a novel positive regulator of ccRCC progression, invasion and metastasis." International journal of cancer 133.12 (2013): 2801-2811.

Author: Available in link provided

Date of Publication : Available in link provided

Effect of Fumonisin B1 on Proliferation and Apoptosis of Intestinal Porcine Epithelial Cells Toxins Tianjie Wang WB Pig

Author: Available in link provided

Date of Publication : Available in link provided

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

Author: Available in link provided

Date of Publication : Available in link provided

Salvianolic acid A alleviates H2O2-induced endothelial oxidative injury via miR-204-5p Scientific Reports Qiao Xilin WB Human 1:1000 human umbilical vein endothelial cell (HUVEC)

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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