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VEGF Receptor 2 (Phospho Tyr1175) (AB5212) Rabbit mAb
VEGF Receptor 2 (Phospho Tyr1175) (AB5212) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0086
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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VEGF Receptor 2 (Phospho Tyr1175) (AB5212) Rabbit mAb

Western blot analysis of lysates from A-431 lysates cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb (M0086) at 1:3000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

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Product Details
Product Name
VEGF Receptor 2 (Phospho Tyr1175) (AB5212) Rabbit mAb
Description
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. . Mutations of this gene are implicated in infantile capillary hemangiomas.
Catalog No
M0086
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho Y1175
Clone
AB5212
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around Y1175 of human VEGF Receptor 2.
Specificity
The antibody detects endogenous levels of VEGF Receptor 2 protein only when phosphorylated at Tyr1175. The antibody does not cross-react with VEGF Receptor 2 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.09% Sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
KDR
Gene ID
3791
Human swiss
P35968
Protein Name
VEGF Receptor 2 (Phospho Tyr1175)
Synonyms
FLK1; CD309; VEGFR; VEGFR2; Phospho-VEGF Receptor 2-Y1175
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Price/Quantity:
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Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
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