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HSF1(Phospho Ser326) (AB3812) Rabbit mAb
HSF1(Phospho Ser326) (AB3812) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0076
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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HSF1(Phospho Ser326) (AB3812) Rabbit mAb

Western blot analysis of lysates from 293T cells, using Phospho-HSF1-S326 Rabbit mAb (M0076) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1min.

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Product Details
Product Name
HSF1(Phospho Ser326) (AB3812) Rabbit mAb
Description
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE) . This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Catalog No
M0076
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho S326
Clone
AB3812
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S326 of human HSF1 (Q00613).
Specificity
The antibody detects endogenous levels of HSF1 protein only when phosphorylated at Ser326. The antibody does not cross-react with HSF1 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
HSF1
Gene ID
3297
Human swiss
Q00613
Protein Name
HSF1(Phospho Ser326)
Synonyms
HSTF1; Phospho-HSF1-S326
Related Products

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HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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