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Primary Antibodies
FOXO1 (Phospho Thr24) + FOXO3A (Phospho Thr32) + FOXO4 (Phospho Thr28) (AB3523) Rabbit mAb
FOXO1 (Phospho Thr24) + FOXO3A (Phospho Thr32) + FOXO4 (Phospho Thr28) (AB3523) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0061
Species Reactivity:Human,Mouse
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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FOXO1 (Phospho Thr24) + FOXO3A (Phospho Thr32) + FOXO4 (Phospho Thr28) (AB3523) Rabbit mAb

Western blot analysis of lysates from Jurkat cells, using Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28 Rabbit mAb (M0061) at 1:2000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

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Product Details
Product Name
FOXO1 (Phospho Thr24) + FOXO3A (Phospho Thr32) + FOXO4 (Phospho Thr28) (AB3523) Rabbit mAb
Description
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
Catalog No
M0061
Species Reactivity
Human, Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T24, Phospho T32, Phospho T28
Clone
AB3523
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:1000-1:5000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T24 & T32 & T28 of human FOXO1/FOXO3A/FOXO4FOXO1.
Specificity
The antibody detects endogenous levels of FOXO1 protein when phosphorylated at Thr24, FOXO3A protein phosphorylated at Thr32, and FOXO4 protein phosphorylated at Thr28.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
FOXO1/FOXO3A/FOXO4
Gene ID
2308; 2309; 4303
Human swiss
Q12778; O43524; P98177
Protein Name
FOXO1 (Phospho Thr24) + FOXO3A (Phospho Thr32) + FOXO4 (Phospho Thr28)
Synonyms
FoxO1/FoxO3a/FoxO4; Phospho-FOXO1-T24 + FOXO3A-T32 + FOXO4-T28
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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