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RIP3 (Phospho Thr231/Ser232) (AB4771) Rabbit mAb
RIP3 (Phospho Thr231/Ser232) (AB4771) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0020
Species Reactivity:Mouse
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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RIP3 (Phospho Thr231/Ser232) (AB4771) Rabbit mAb

Western blot analysis of lysates from L-929, using Phospho-RIP3-T231/S232 Rabbit mAb (M0020) at 1:700 dilution.L-929 cells were treated by 20 ng/ml mouse TNF-a, 100 nM BV-6 and 20 μM z-VAD for 5 or 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

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Product Details
Product Name
RIP3 (Phospho Thr231/Ser232) (AB4771) Rabbit mAb
Description
Enables NF-kappaB-inducing kinase activity and protein serine/threonine kinase activity. Involved in several processes, including execution phase of necroptosis; hematopoietic or lymphoid organ development; and regulation of programmed cell death. Acts upstream of or within several processes, including I-kappaB kinase/NF-kappaB signaling; positive regulation of intrinsic apoptotic signaling pathway; and positive regulation of protein deacetylation. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; hemolymphoid system gland; male reproductive gland or organ; primitive streak; and turbinate bone primordium. Orthologous to human RIPK3 (receptor interacting serine/threonine kinase 3) .
Catalog No
M0020
Species Reactivity
Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T231/S232
Clone
AB4771
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T231 & S232 of mouse RIP3.
Specificity
The antibody detects endogenous levels of RIP3 protein only when phosphorylated at Thr231/Ser232. The antibody does not cross-react with RIP3 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
Ripk3
Gene ID
56532
Human swiss
Q9QZL0
Protein Name
RIP3 (Phospho Thr231/Ser232)
Synonyms
Rip3; 2610528K09Rik; Phospho-RIP3-T231/S232
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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