25% Off Select Antibodies and ELISA Kits
Sign In/Register 0

We use cookies, check our Privacy Policies.

Products
Primary Antibodies
HIF-1alpha (AB3722) Rabbit mAb
HIF-1alpha (AB3722) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0005
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
#
#

HIF-1alpha (AB3722) Rabbit mAb

Western blot analysis of lysates from HeLa cells, using HIF-1alpha Rabbit mAb (M0005) at 1:1000 dilution.HeLa cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

Delivery
Contact Us
Telephone
408-747-0185
Fax
408-747-0145
Email
order@assaybiotech.com
Promotions
Product Details
Product Name
HIF-1alpha (AB3722) Rabbit mAb
Description
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1) , which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Catalog No
M0005
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB3722
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 600 - 700 of human HIF1α.
Specificity
This antibody detects endogenous levels of HIF-1alpha protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
HIF1A
Gene ID
3091
Human swiss
Q16665
Protein Name
HIF-1alpha
Synonyms
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
    © 2025 AssayBiotech. All Rights Reserved.
    Follow Us
    Terms of Use Privacy Policy