Taq Visual SYBR qPCR Premix (Universal) is a qPCR reagent specifically designed for SYBR Green I intercalating dye-based assays. This 2× premix includes all necessary qPCR components except for primers and DNA samples, thereby reducing operational steps, shortening sample loading time, and minimizing the risk of contamination. Its core component is an antibody-modified hot-start Taq DNA polymerase, combined with a meticulously optimized buffer system and PCR reaction enhancers. This formulation provides strong specificity and high amplification efficiency, effectively inhibiting non-specific amplification and enabling accurate quantification of templates across a wide concentration range, resulting in stable and reliable qPCR results. This product already contains a universal reference dye and is compatible with most qPCR instruments, eliminating the need for additional dyes for instrument calibration. This product utilizes the color-changing effect resulting from the mixing of different dyes to track the process, thereby significantly minimizing pipetting errors. Taq Visual SYBR qPCR Premix contains a blue dye, while the 10× Dilution Buffer contains a yellow dye. When Taq Visual SYBR qPCR Premix (blue) is mixed with template diluted in Dilution Buffer (yellow), a color change from blue to green occurs, allowing for an accurate visual confirmation of template addition based on the liquid color.