T4 DNA Ligase (Fast)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: YS0082

Concentration:5 U/μl

Size:

Price/Quantity $91

Quantity:

Datasheet

T4 DNA Ligase (Fast)

Molecular Biology Products by Assay Biotechnology Inc.

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Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

T4 DNA Ligase (Fast)

Description

T4 DNA Ligase(Fast) is produced by E.coli carrying T4 bacteriophage gene 30. The enzyme catalyzes the formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl groups of double-stranded DNA or RNA. It can repair single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrids and can ligate DNA fragments with sticky or blunt ends. It is not active on single-stranded nucleic acids. The enzyme is primarily used for cloning of restriction endonuclease-digested DNA fragments, site-directed mutagenesis, cloning of PCR products, linear DNA circularization, and repair of double-stranded DNA nicks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes sticky end ligation reactions at room temperature in just 10 minutes.

Catalog No

YS0082

Application

Mainly used for cloning DNA fragments generated by restriction endonuclease digestion, site-directed mutagenesis, PCR product cloning, repair of double-stranded DNA gaps, and linear DNA self-circularization.

Properties

High purity, precise enzyme activity, and efficient ligation.