Description
Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an Extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divi
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EFNA5; EPLG7; LERK7; Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7
Molecular Weight (Da)
26297
Gene Name
EFNA5
Protein Name
Ephrin-A5
Human Gene ID
1946
Human Swiss Prot No.
P52803
Immunogen
Synthesized peptide derived from Ephrin-A5 . at AA range: 130-210
Specificity
Ephrin-A5 Polyclonal Antibody detects endogenous levels of Ephrin-A5 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
