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Primary Antibodies
Iba 1 (PTR1347) Antibody
Iba 1 (PTR1347) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: W0245
Reactivity:Human,Mouse,Rat
Applications:IHC,WB,IF,ELISA
Modification:Unmodified/Total
Source:Mouse,Monoclonal/IgG1,kappa
Storage and Stability:-15°C to -25°C/1 year(Do not lower than -25°C)

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Immunohistochemical
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Iba 1 (PTR1347) Antibody

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit (RS0038, Assay Biotech). CK-pan mouse mAb(V0280 Assay Biotech) green, Ki-67 rabbit mAb(YM7002 Assay Biotech) red, Iba 1 mouse mAb(W0245 Assay Biotech) purple,CD8 a mouse mAb(W0296 Assay Biotech) cyan, CD20 mouse mAb(W0276 Assay Biotech) yellow, The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Assay Biotech YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

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Product Details
Product Name
Iba 1 (PTR1347) Antibody
Description
Catalog No
W0245
Reactivity
Human, Mouse, Rat
Applications
IHC, WB, IF, ELISA
Modification
Unmodified/Total
Source
Mouse, Monoclonal/IgG1, kappa
Dilution
IHC 1:200-1000. WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
Purification
Protein G
Storage and Stability
-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name
AIF 1; AIF-1; Aif1; AIF1 protein; AIF1_HUMAN; Allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; allograft inflammatory factor-1 splice variant Hara-1; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Interferon responsive transcript 1; interferon responsive transcript factor 1; Ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein g1;
Molecular Weight (Da)
16kD
Gene Name
AIF1 G1 IBA1
Protein Name
Allograft inflammatory factor 1 (AIF-1) (Ionized calcium-binding adapter molecule 1) (Protein G1)
Human Gene ID
199
Human Swiss Prot No.
P55008
Immunogen
Synthesized peptide derived from part region of human protein AA range: 50-147
Specificity
This antibody detects endogenous levels of Iba 1 protein.
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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