NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the Cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Catalog No
D12819
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Meythl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Molecular Weight (Da)
60219
Gene Name
RELA
Protein Name
Transcription factor p65
Human Gene ID
5970
Human Swiss Prot No.
Q04206
Immunogen
Synthesized peptide derived from human p65 around the mono-methylation site of K314/K315.
Specificity
Mono-Methyl-NFκB-p65 (K314/K315) Polyclonal Antibody detects endogenous levels of p65 protein only when mono-methylated at K314/K315.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western Blot analysis of MCF7 cells using Mono-Methyl-NFκB-p65 (K314/K315) Polyclonal Antibody. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
