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Primary Antibodies
β-Tubulin Monoclonal Antibody(5G3)
β-Tubulin Monoclonal Antibody(5G3)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: N1294
Reactivity:Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Sheep,Insect,Yeast,Fish
Applications:WB,IF/ICC,IHC-p
Modification:Unmodfied/ Total
Source:Monoclonal Mouse
Storage and Stability:-20°C/1 year

$184
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β-Tubulin Monoclonal Antibody(5G3)

Western blot analysis of A549 (1). Rat brain (2). Mouse brain (3). Chicken lung (4) and Rabbit testis (5). Sheep muscle (6). diluted at 1:5000.

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Product Details
Product Name
β-Tubulin Monoclonal Antibody(5G3)
Description
tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],
Catalog No
N1294
Reactivity
Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Sheep,Insect,Yeast,Fish
Applications
WB,IF/ICC,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:5000 IHC: 1:200 IF 1:200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Molecular Weight (Da)
50433
Gene Name
TUBB3
Protein Name
Tubulin beta-3 chain
Human Gene ID
10381
Human Swiss Prot No.
Q13509
Immunogen
Synthetic Peptide of β-Tubulin
Specificity
The antibody detects endogenous β-tubulin protein.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Publications (39)
Exosomal thioredoxin-1 from hypoxic human umbilical cord mesenchymal stem cells inhibits ferroptosis in doxorubicin-induced cardiotoxicity via mTORC1 signaling

Author: Available in link provided

Date of Publication : Available in link provided

Hsa_circ_0001550 impairs decidualization by regulating the proliferation and apoptosis of endometrial stromal cells

Author: Available in link provided

Date of Publication : Available in link provided

EMT status of circulating breast cancer cells and impact of fluidic shear stress

Author: Available in link provided

Date of Publication : Available in link provided

Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology

Author: Available in link provided

Date of Publication : Available in link provided

Large-scale chromatin reorganization reactivates placenta-specific genes that drive cellular aging WB Human /hMPCs

Author: Available in link provided

Date of Publication : Available in link provided

HOXC6 Regulates the Epithelial-Mesenchymal Transition through the TGF-β/Smad Signaling Pathway and Predicts a Poor Prognosis in Glioblastoma WB Human glioblastoma/U251 cell, U87 cell, A172 cell, T98G cell, H4 cell, SHG44 cell

Author: Available in link provided

Date of Publication : Available in link provided

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:1000 retinal tissue

Author: Available in link provided

Date of Publication : Available in link provided

KLF2 up-regulates IRF4/HDAC7 to protect neonatal rats from hypoxic-ischemic brain damage Cell Death Discov. 2022 Jan;8(1):1-10. IF Rat 1:200 cerebral cortex

Author: Available in link provided

Date of Publication : Available in link provided

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB Mouse 1:1000 retina

Author: Available in link provided

Date of Publication : Available in link provided

SIRT3 consolidates heterochromatin and counteracts senescence. NUCLEIC ACIDS RESEARCH Nucleic Acids Res. 2021 May;49(8):4203-4219 WB Human hMSCs

Author: Available in link provided

Date of Publication : Available in link provided

CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2019 Feb;54(2):585-599 WB Human 1:5000 5-8F cell

Author: Available in link provided

Date of Publication : Available in link provided

Protective Mechanism of Adipose-Derived Stem Cells in Remodelling of the Skin Stem Cell Niche During Photoaging. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Dec 11 WB Mouse 1:10000 skin tissues

Author: Available in link provided

Date of Publication : Available in link provided

Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway. PHYTOMEDICINE Phytomedicine. 2021 Aug;89:153599 WB Human 1 : 1000 Lung A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

Label-free proteomic strategy to compare the proteome differences between longissimus lumborum and psoas major muscles during early postmortem periods. FOOD CHEMISTRY 2018 Jul 06 WB Bovine 1: 5000 M. longissimus lumborum (LL), M. psoas major (PM)

Author: Available in link provided

Date of Publication : Available in link provided

Modulation of TRIB3 and Macrophage Phenotype to Attenuate Insulin Resistance After Downhill Running in Mice. Frontiers in Physiology Front Physiol. 2021; 12: 637432 WB Mouse Adipose

Author: Available in link provided

Date of Publication : Available in link provided

Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2019 Jan;20(16):3921 WB Bovine 1:5000 sperm

Author: Available in link provided

Date of Publication : Available in link provided

Heparanase from triple‑negative breast cancer and platelets acts as an enhancer of metastasis. INTERNATIONAL JOURNAL OF ONCOLOGY 2020 Aug 27 WB Human 1 : 10000 MDA-MB-231 cell

Author: Available in link provided

Date of Publication : Available in link provided

Stress aggravates and prolongs imiquimod‐induced psoriasis‐like epidermal hyperplasis and IL‐1β/IL‐23p40 production. JOURNAL OF LEUKOCYTE BIOLOGY J Leukocyte Biol. 2020 Jul;108(1):267-281 WB Mouse 1:10000 Skin

Author: Available in link provided

Date of Publication : Available in link provided

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2019 Apr;112:108669 WB Mouse Primary murine lung fibroblasts (PMLFs)

Author: Available in link provided

Date of Publication : Available in link provided

Liver macrophages mediate effects of downhill running and caloric restriction on nonalcoholic fatty liver disease of high fat diet-fed mice. LIFE SCIENCES 2020 Jun 15 WB Mouse Liver

Author: Available in link provided

Date of Publication : Available in link provided

CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway. Journal of Cancer J Cancer. 2020; 11(2): 272–283 WB Human 1:5000 JEG-3 cell

Author: Available in link provided

Date of Publication : Available in link provided

Acetyl-11-keto-β-boswellic acid inhibits the secretion of cytokines by dendritic cells via the TLR7/8 pathway in an imiquimod-induced psoriasis mouse model and in vitro. LIFE SCIENCES 2018 May 31 WB Mouse 1:5000 Skin BMDCs

Author: Available in link provided

Date of Publication : Available in link provided

Peptides derived from rice α-globulin reduce atherosclerosis in apolipoprotein E-deficient mice by inhibiting TNF-α-induced vascular endothelial cells injury. Journal of Functional Foods 2019 Sep 20 WB Human 1:5000 HUVECs

Author: Available in link provided

Date of Publication : Available in link provided

Substrate stiffness affects the morphology, proliferation, and radiosensitivity of cervical squamous carcinoma cells. TISSUE & CELL Tissue Cell. 2022 Feb;74:101681 WB，IF Human SiHa cell

Author: Available in link provided

Date of Publication : Available in link provided

Pollen developmental defects in ZD-CMS rice line explored by cytological, molecular and proteomic approaches. Journal of Proteomics 2014 May 28 WB Rice florets

Author: Available in link provided

Date of Publication : Available in link provided

Influence of the total saponin fraction from Dioscorea nipponica Makino on TLR2/4-IL1R receptor singnal pathway in rats of gouty arthritis. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2017 Jul;206:274 WB Rat 1: 5000 sunovial tissue

Author: Available in link provided

Date of Publication : Available in link provided

Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2017 Mar 21 WB Mouse Skin

Author: Available in link provided

Date of Publication : Available in link provided

Relationship between coronary artery calcification and calcium deposition in the myocardium:. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 2019 May 22 WB Human myocardial tissue

Author: Available in link provided

Date of Publication : Available in link provided

Qilin pills alleviate oligoasthenospermia by inhibiting Bax-caspase-9 apoptosis pathway in the testes of model rats. Oncotarget Oncotarget. 2018 Apr 24; 9(31): 21770–21782 WB Rat 1:5000 testis tissue

Author: Available in link provided

Date of Publication : Available in link provided

Anti-Inflammation and Anti-Pyroptosis Activities of Mangiferin via Suppressing NF-κB/NLRP3/GSDMD Signaling Cascades INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Shidong Zhang WB Mouse

Author: Available in link provided

Date of Publication : Available in link provided

Amelioration of Lung Fibrosis by Total Flavonoids of Astragalus via Inflammatory Modulation and Epithelium Regeneration AMERICAN JOURNAL OF CHINESE MEDICINE Min Li WB Human,Mouse lung tissues A549 cells

Author: Available in link provided

Date of Publication : Available in link provided

Ceramide Synthase 1 Inhibits Brain Metastasis of Non-Small Cell Lung Cancer by Interacting with USP14 and Downregulating the PI3K/AKT/mTOR Signaling Pathway Cancers Hongru Li WB Human benign prostatic hyperplasia (BPH) tissue H1299 cell,PC-9 cell

Author: Available in link provided

Date of Publication : Available in link provided

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells CELLULAR ONCOLOGY Li Jun WB Human BGC823 cell,MKN28 cell

Author: Available in link provided

Date of Publication : Available in link provided

MAOA suppresses the growth of gastric cancer by interacting with NDRG1 and regulating the Warburg effect through the PI3K/AKT/mTOR pathway. Li Jun WB Human 1:5000 BGC-823 cell,HGC-27 cell

Author: Available in link provided

Date of Publication : Available in link provided

Regulation of secondary hair follicle cycle in cashmere goats by miR-877-3p targeting IGFBP5 gene. JOURNAL OF ANIMAL SCIENCE Xuefeng Fu WB Goat 1:10000 dermal papilla cell

Author: Available in link provided

Date of Publication : Available in link provided

Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice Nutrients Tengteng Ji WB Mouse 1:10000 liver tissue,adipose tissue

Author: Available in link provided

Date of Publication : Available in link provided

Novel sulfonyl-substituted tetrandrine derivatives for colon cancer treatment by inducing mitochondrial apoptosis and inhibiting PI3K/AKT/mTOR pathway BIOORGANIC CHEMISTRY Jie Ling WB Human,Mouse HCT116 cell,CT26 cell

Author: Available in link provided

Date of Publication : Available in link provided

MnO2 nanozyme with lanthanide-based radiosensitization for advanced radiotherapy by tumor microenvironment triggering STING pathway activation CHEMICAL ENGINEERING JOURNAL Meiling Tan WB Mouse CT26 cell

Author: Available in link provided

Date of Publication : Available in link provided

Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway BIOFACTORS Yining Lei WB Rat 1:5000 Hippocampal tissue

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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