The Tfact Fos TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of Fos active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
Catalog No
TFE-7013
Format
1 Plate: 12 x 8-Well Microstrips
Detection Target
Fos
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
DNA-Binding ELISA
SwissProt ID
P01100
NCBI Gene ID
FOS
Target Synonyms
Cellular oncogene fos; FOS; G0/G1 switch regulatory protein 7; G0S7; Proto-oncogene protein c-fos
The TFact™ Fos DNA-Binding ELISA detects active Fos in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stablization buffer in the Jurkat nuclear extract with PMA stimulation.
