The Tfact SPI1 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of SPI1 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ SPI1 DNA-Binding ELISA detects active SPI1 in Hela Nuclear Extract. The Hela cells were grown 3 days in RPMI 1640 and DMEM with 10% FBS and harvested for nuclear extract. Jurkat cells were stimulated by PMA (200nM) before harvest.
