The Tfact GATA1 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of GATA1 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ GATA1 DNA-Binding ELISA detects active GATA1 in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells are stimulated by PMA (200nM) or directly harvested without PMA stimulation. The DNA-transcription factor complex is treated with stablization buffer,