The Tfact SRF TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of SRF active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ SRF DNA-Binding ELISA detects active SRF in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by PMA (200nM) before harvest.
