The DDR1 (Phospho-Tyr513) Phospho Sandwich ELISA Kit allows for qualitative detection of DDR1 (Phospho-Tyr513) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HepG2 cells were grown to 90% confluency and were stimulated with Vanadate 1mM and incubated for 30 mins. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of DDR1 P-Tyr513 versus untreated HepG2 lysates.