IL-8 (Human) OmniKine™ ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: OK-0142

Reactivity:Human

Assay Type:Sandwich ELISA

Format:1 plate: 12 x 8-Well Microstrips

Dynamic Range:16-1000 pg/mL

Size:

Price/Quantity $310

Quantity:

Datasheet

SDS

Recovery and Linearity 80-120%

Sample Specific Diluent

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IL-8 (Human) OmniKine™ ELISA Kit

The data and subsequent graph were obtained after performing a cytokine ELISA for IL-8. Each known sample concentration was assayed in triplicate. The OmniKine™ Human IL-8 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-8 proteins within the range of 16-1000 pg/ml.

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Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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408-747-0145

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Product Details

Product Name

IL-8 (Human) OmniKine™ ELISA Kit

Description

The IL-8 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natural or recombinant IL-8 concentrations within any experimental sample including cell lysates, serum and plasma.

Catalog No

OK-0142

Format

1 plate: 12 x 8-Well Microstrips

Detection Target

IL-8

Reactivity

Human

Recovery Data

Three concentrations of recombinant Human IL-8 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate. The average recovery for plasma, serum and RPMI FBS 10% were: 84, 94, 86.

Linearity Data

Human IL-8 recombinant protein was spiked into various biological samples and serial diluted with sample specific diluent for serum and/or plasma samples and RPMI FBS10%. The average linearities for serum, plasma and RPMI FBS10% were as follows: 84.75, 89.5, 92.

Detection Method

Colorimetric 450 nm

Assay Type

Sandwich ELISA

SwissProt ID

P10145

NCBI Gene ID

3576

Target Synonyms

Interleukin-8, C-X-C motif chemokine 8, Emoctakin, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, Monocyte-derived neutrophil-activating peptide, MONAP, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, T-cell chemotactic factor, CXCL8

Storage and Stability Guarantee

4°C/3 Months

Sample Type

Plasma, Serum, Cell Culture Supernatant

Range

16-1000 pg/mL

Assay Time

4.5 hours