The Notch-1 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Notch-1 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0257
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
Notch-1
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P46531
NCBI Gene ID
4851
Target Synonyms
Neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1
Human Notch-1 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Notch-1 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Notch-1. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Notch-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Notch-1 proteins within the range of 79-5000 pg/ml.