notch 1(NOTCH1) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymph
Catalog No
L0119
Reactivity
Human, Mouse, Rat
Applications
WB, IF/ICC, IHC-p, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1
Molecular Weight (Da)
272500
Gene Name
NOTCH1
Protein Name
Neurogenic locus notch homolog protein 1
Human Gene ID
4851
Human Swiss Prot No.
P46531
Immunogen
The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784
Specificity
Cleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using Notch 1 (Cleaved-Val1754) Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Notch 1 (Cleaved-Val1754) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Qu, Xiaochen, et al. "MiR‐199b‐5p inhibits osteogenic differentiation in ligamentum flavum cells by targeting JAG1 and modulating the Notch signalling pathway." Journal of cellular and molecular medicine 21.6 (2017): 1159-1170.
