The Galectin-3 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Galectin-3 concentrations within any experimental sample including cell lysates, serum and plasma.
Human Galectin-3 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent NM33 for serum samples and Assay Diluent LN28 for RPMI 10% FBS.
Three concentrations of recombinant Human Galectin-3 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Galectin-3. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Galectin-3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Galectin-3 proteins within the range of 63-4000 pg/ml.
