The HGF (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant HGF concentrations within any experimental sample including cell lysates, serum and plasma.
Human HGF recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum samples and Assay Diluent 1TD for RPMI 10% FBS.
The data and subsequent graph were obtained after performing a cytokine ELISA for HGF. Each known sample concentration was assayed in triplicate. The OmniKine™ Human HGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human HGF proteins within the range of 125-8000 pg/ml.