The ER81 Colorimetric Cell-Based ELISA is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ER81 protein expression profile in cells. The kit can be used for measuring the relative amounts of ER81 in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on ER81.
Western blot analysis of extracts from HuvEc cells, treated with PMA 125ng/ml 30', using ER81 Antibody. The lane on the right is treated with the synthesized peptide.