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Phospho-RSK1/2/3/4 (Ser221/227/S218/232) Colorimetric Cell-Based ELISA Kit
Phospho-RSK1/2/3/4 (Ser221/227/S218/232) Colorimetric Cell-Based ELISA Kit
Catalog No :
CBP1772
Reactivity
H:S221/227/S218/232,M:S221/227/S218/252
Assay Type
Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-RSK1/2/3/4 (Ser221/227/S218/232) Colorimetric Cell-Based ELISA Kit
Description
The Phospho-RSK1/2/3/4(Ser221/227/S218/232) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-RSK1/2/3/4(Ser221/227/S218/232) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-RSK1/2/3/4(Ser221/227/S218/232) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-RSK1/2/3/4(Ser221/227/S218/232).
Catalog No
CBP1772
Format
2 Plates: TC 96-Well Plates
Detection Target
Phospho-RSK1/2/3/4(Ser221/227/S218/232)
Reactivity
H:S221/227/S218/232, M:S221/227/S218/252
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
Q15418/P51812/Q15349/Q9UK32
NCBI Gene ID
6195/6197/6196/27330
Target Synonyms
S6K-alpha 1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; Ribosomal S6 kinase 1; RSK-1; pp90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPKAPK1A/ S6K-alpha 3; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; Ribosomal S6 kinase 2; RSK-2; pp90RSK2; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPKAPK1B/ S6K-alpha 2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; Ribosomal S6 kinase 3; RSK-3; pp90RSK3; MAP kinase-activated protein kinase 1c; MAPKAPK1C/ S6K-alpha 6; 90 kDa ribosomal protein S6 kinase 6; p90-RSK 6; Ribosomal S6 kinase 4; RSK-4; pp90RSK4
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Western blot analysis of extracts from Jurkat cells
Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30', using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody.
