Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]
Catalog No
D0002
Reactivity
Human, Mouse, Rat
Applications
IHC-p, IF(paraffin section), WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
H2AFZ; H2AZ; Histone H2A.Z; H2A/z
Molecular Weight (Da)
51633
Gene Name
H2AFZ
Protein Name
Histone H2A.Z
Human Gene ID
3015
Human Swiss Prot No.
P0C0S5
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2A around the acetylated site of Lys5. AA range:1-50
Specificity
Acetyl-Histone H2A (K5) Polyclonal Antibody detects endogenous levels of Histone H2A protein only when acetylated at K5.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
