Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
Catalog No
D12090
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK21AC
Molecular Weight (Da)
Gene Name
Histone H2B
Protein Name
Histone H2B
Human Gene ID
255626
Human Swiss Prot No.
Q96A08/P33778/P62807
Immunogen
Synthetic Acetyl peptide from human protein at AA range: 21
Specificity
The antibody detects endogenous Histone H2B when Acetyl occurs at Lys21
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of 3T3 mouse-kidney KB K562 Hela lysate, antibody was diluted at 2000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
