This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Catalog No
D12102
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1; YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; SFN; HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin
Molecular Weight (Da)
Gene Name
YWHAB/YWHAG/YWHAQ/YWHAZ/SFN
Protein Name
14-3-3 protein beta/alpha/14-3-3 protein gamma/14-3-3 protein theta/14-3-3 protein zeta/delta/14-3-3 protein sigma
Human Gene ID
7529
Human Swiss Prot No.
P31946
Immunogen
Synthesized acetyl-peptide derived from human 14-3-3-pan around the acetylation site of K51.
Specificity
Acetyl-14-3-3-pan (K51/49) Polyclonal Antibody detects endogenous levels of 14-3-3-pan around the acetylation site of K51 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of MOUSE-SPLEEN using Acetyl-14-3-3-pan (K51/49) antibody. Antibody was diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
