This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Catalog No
D12124
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1
Molecular Weight (Da)
24894
Gene Name
HMGB1
Protein Name
High mobility group protein B1
Human Gene ID
3146
Human Swiss Prot No.
P09429
Immunogen
Synthesized acetyl-peptide derived from the N-terminal region of human HMG-1 around the acetylation site of K12.
Specificity
Acetyl-HMG-1 (K12) Polyclonal Antibody detects endogenous levels of HMG-1 protein only when acetylation at K12.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western Blot analysis of HepG2 cells treated with UV using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
