The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
Catalog No
D12129
Reactivity
Human:K127/79, Mouse:K127/79, Rat:K127
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) Hepatocyte nuclear factor 4-gamma (HNF-4-gamma) (Nuclear receptor subfamily 2 group A member 2)(TCF-14) (Transcription factor HNF-4)
Molecular Weight (Da)
Gene Name
HNF4A HNF4 NR2A1 TCF14 HNF4G NR2A2
Protein Name
Hepatocyte nuclear factor 4-alpha/gamma (HNF-4-alpha/gamma) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) (TCF-14) (Transcription factor HNF-4)
Human Gene ID
3172
Human Swiss Prot No.
P41235/Q14541
Immunogen
Synthetic Acetyl peptide from human protein at AA range: 127(HNF-4α)/79(HNF-4γ)
Specificity
The antibody detects endogenous HNF-4α/γ when Acetyl occurs at Lys127(alpha)/79(gamma)
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of mouse-lung mouse-brain mouse-heart Hela mouse-liver lysate, antibody was diluted at 500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
