This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the Cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Catalog No
D12130
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
Molecular Weight (Da)
70052
Gene Name
HSPA1A
Protein Name
Heat shock 70 kDa protein 1A/1B
Human Gene ID
3303/3304
Human Swiss Prot No.
P08107
Immunogen
Synthesized acetyl-peptide derived from the Internal region of human HSP70 around the acetylation site of K246.
Specificity
Acetyl-HSP70 (K246) Polyclonal Antibody detects endogenous levels of HSP70 protein only when acetylation at K246.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western Blot analysis of RAT-kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Western Blot analysis of rat kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
