The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
Catalog No
D12146
Reactivity
Human, Rat, Mouse
Applications
IHC-p, IF(paraffin section), WB
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
IHC-p 1:50-200, WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
X-ray repair cross-complementing protein 5 (EC 3.6.4.-) (86 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (CTC box-binding factor 85 kDa subunit) (CTC85) (CTCBF) (DNA repair protein XRCC5) (Ku80) (Ku86) (Lupus Ku autoantigen protein p86) (Nuclear factor IV) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining))
Molecular Weight (Da)
Gene Name
XRCC5 G22P2
Protein Name
Ku-80 (Acetyl-Lys565)
Human Gene ID
7520
Human Swiss Prot No.
P13010
Immunogen
Synthesized peptide derived from human Ku-80 (Acetyl-Lys565)
Specificity
This antibody detects endogenous acetyl levels of Ku-80 (Acetyl-Lys565) at Human:K565
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.