E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Catalog No
D12166
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300
Molecular Weight (Da)
Gene Name
EP300
Protein Name
Histone acetyltransferase p300
Human Gene ID
2033
Human Swiss Prot No.
Q09472
Immunogen
Synthesized acetyl-peptide derived from human p300 around the acetylation site of K1558.
Specificity
Acetyl-p300 (K1558/K1560) Polyclonal Antibody detects endogenous levels of p300 around the acetylation site of K1558 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of HELA 293T using Acetyl-p300 (K1558/K1560) antibody. Antibody was diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000